QUIN 2, Tetrapotassium Salt-CAS 73630-23-6-Calbiochem

C₂₆H₂₃N₃O₁₀ · 4K

693.87

MFCD00065487

Binding of Ca²⁺ to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Spectral properties in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) differ from those during high calcium conditions (ex. 332 nm, em. 505 nm).
Binding of Ca²⁺ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells.

Primary Target
Calcium Probes
Product does not compete with ATP.
Reversible: no
Cell permeable: no

Toxicity: Irritant (B)

Following reconstitution, aliquot and freeze (-20°C) under an inert gas, pH >10. Stock solutions are stable for up to 6 months at -20°C.

Komada, H., et al. 1989. Cell Struct. Funct. 14, 141.
Vijayaraghavan, S. and Hoskins, D. 1989. Cell Calcium10, 241.
Arslan, P., et al. 1985. J. Biol. Chem.260, 2719.
Bryant, D.T. 1985. Biochem. J.226, 613.
Grynkiewicz, G., et al. 1985. J. Biol. Chem.260, 3440.
Ashley R.H., et al. 1984. Biochem. J.219, 149.
Tsien, R.Y., et al. 1982. J. Cell Biol.94, 325.
Tsien, R.Y. 1980. Biochemistry19, 2396.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

yellow

water: 10 mg/mL

λ_ex 332 nm (high)
λ_ex 354 nm (low)
λ_em 505 nm (high)
λ_em 510 nm (low)

ambient

−20℃

1S/C26H27N3O10.4K/c1-15-3-6-19(28(10-22(30)31)11-23(32)33)21(7-15)39-14-17-5-4-16-8-18(38-2)9-20(26(16)27-17)29(12-24(34)35)13-25(36)37;;;;/h3-9H,10-14H2,1-2H3,(H,30,31)(H,32,33)(H,34,35)(H,36,37);;;;/q;4*+1/p-4

XAGUNWDMROKIFJ-UHFFFAOYSA-J

50 mg in Plastic ampoule

11 - Combustible Solids

WGK 3