PKCε, Human, Recombinant, S. frugiperda

≥85% (SDS-PAGE)

100

liquid

≥800 units/mg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

20 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Full-length, recombinant, human, PKCε expressed in S. frugiperda insect cells using a baculovirus expression system. Suitable for studies of PKCε and its regulation. PKCε is a Ca²⁺-independent and phospholipid-dependent enzyme.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to PKC_ε Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.4.

In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON^™ X-100 Detergent, pH 7.4.

Following initial thaw, aliquot and freeze (-70°C).

Newton, A.C. 1995. J. Biol. Chem.270, 28495.
Dekker, L.V., and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.
Nishizuka, Y. 1992. Science 258, 607.
Bell, R.M., and Burns, D.J. 1991. J. Biol. Chem.266, 4661.

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11 - Combustible Solids

WGK 1