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Magna ChIP® HiSens Chromatin Immunoprecipitation Kit

产品编号:4157863
包装规格:24 ASSAYS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
415786324 ASSAYS6990
产品性质
Quality Level【质量水平】
100
packaging【包装】
Kit capacity: 24 chromatin immunoprecipitation assays
基本信息
General description【一般描述】
The Magna ChIP HiSens kit provides a complete set of validated, quality controlled reagents, and a detailed protocol to enable ChIP from a wide range of input amounts of chromatin obtained from either cells or tissues. Our specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipiptation and enables the use of a broader range of antibodies than protein A or G alone eliminating the need to purchase different kits for different antibody isotypes. The SCW Buffer is unique to the Magna ChIP HiSens kit and enables the use of a single buffer for multiple steps of the ChIP process (sonication, chromatin immunoprecipitation, and wash). The ChIP elution buffer provided with the HiSens kit has been formulated to allow analysis of enrichment by qPCR without additional clean-up steps for more rapid results.
Application【应用】
Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to enable ChIP from low input amounts of chromatin obtained from either cells or tissues using magnetic A/G beads.
Features and Benefits【特点和优势】
  • Robust ChIP from as few as 10,000 to as many as 1,000,000 cells Protocols and reagents for generation of chromatin from range of input sample type.
  • Superior performance with variety of isotypes of either polyclonal or monoclonal antibodies
  • Specialized buffer and bead formulations; lower backgrounds and higher fold enrichment.
  • Single buffer system for sonication, chromatin IP, and wash
  • Perform analysis of enrichment without additional purification after cross link reversal.
  • Compatible with all commonly used downstream analysis applications– qPCR, next generation sequencing, microarray.

Components【组分】
10X Glycine;Nuclei Isolation Buffer;10X PBS;SCW Buffer (Sonication/ChIP/Wash);Magna ChIP Protein A/G Magnetic Beads;Low Stringency IP Wash Buffer;ChIP Elution Buffer;Protease Inhibitor Cocktail;Proteinase K Solution
Legal Information【法律信息】
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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