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HybridSPE® DPX Tip

产品编号:4157415
规格:weight 50 mg (bed), Hamilton, tip volume 1 mL
包装规格:96 TIPS
产品类别:进口试剂
品牌:Supelco
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
415741596 TIPS2770
产品别名

HybridSPE® DPX Tip

HybridSPE DPX SPE Tips

产品性质
product line【产品线】
HybridSPE®
composition【组成】
HybridSPE® sorbent
packaging【包装】
box of 96 tips
technique(s)
solid phase extraction (SPE): suitable
tip volume【吸头体积】
1 mL
weight【重量】
50 mg (bed)
matrix active group【基质活性基团】
Zirconia-based phase
application(s)
food and beverages
compatibility【相容性】
Hamilton
基本信息
General description【一般描述】
DPX stands for Dispersive Pipette EXtraction. HybridSPE DPX Tips are pipette tips that incorporate loosely contained HybridSPE sorbent material that is mixed with the sample solution to accomplish solid phase extraction. HybridSPE technology is a simple and generic sample prep platform designed for the gross level removal of endogenous phospholipid interferences from biological plasma and serum prior to LC/MS or LC/MS/MS analysis.

In this simple technique, biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is extracted using the HybridSPE DPX tip which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids.

The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE® stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC/MS or LC/MS/MS analysis.

We offer an array of tips that are compatible with the most common liquid handling platforms and pipettors on the market: Tecan, Hamilton, INTEGRA, and a Universal format as well.

What size tips do I need?
There are two versions of the DPX tips available for the HybridSPE-PL. The standard HybridSPE DPX 50 mg tips are designed for use with biological sample volumes between 100-300 μL, while the HybridSPE DPX 30 mg tips are designed for use with biological sample volumes between 30-100 μL.

Guideline for 30 mg tips - plasma/serum sample: 30-100 μL; precipitating agent: 90-300 μL
Guideline for 50 mg tips - plasma/serum sample: 100-300 μL; precipitating agent: 300-900 μL
Features and Benefits【特点和优势】
  • Minimal elution solvent volumes
  • Rapid extraction times (less than 3 minutes/sample)
  • High extraction efficiencies
  • Easy to perform extractions
  • Lower costs
  • Higher throughput
  • Minimal training required
  • Environmentally friendly
  • Eliminates ion suppression effects often associated with phospholipids
  • Tips offered to fit many formats: Tecan, Hamilton®, INTEGRA, and Universal liquid handling platforms and pipettors.
Legal Information【法律信息】
Hamilton is a registered trademark of Hamilton Co.
HybridSPE is a registered trademark of sigma-aldrich Co. LLC
安全信息
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 3
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