HPTLC Silica gel F₂₅₄ GLP

glass support
silica gel 60 matrix

100

binder Organic Polymer
fluorescent indicator

pkg of 25 plates

thin layer chromatography (TLC): suitable

200 μm

20 cm × 10 cm

5-6 μm

60 Å medium pore diameter

2-30℃

These plates feature individual laser coding for GLP applications. Designed for GLP applications, the tops of each of these plates bear an item, batch, and individual plate number. As a result, lab professionals can easily record and archive every plate they use. Based on our proven silica found in TLC and HPTLC plates, these GLP plates deliver equally unsurpassed separation performance.
25 Glass plates 20 x 10 cm

Glass HPTLC Silica gel F₂₅₄ GLP plates. Silica gel HPTLC plates size 20 x 10 cm, 25 sheets.

Replaces: 5613-6; 5613

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steriod test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)