Dkk-1, Mouse, Recombinant

100

expressed in CHO cells

≥95% (SDS-PAGE)

liquid

Calbiochem^®

OK to freeze

<1.0 EU/μg protein Endotoxin

ambient

−20℃

10 μg in Glass bottle

Recombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND₅₀ = 0.02-0.1 µg/ml.

ND₅₀ = 0.02-0.1 µg/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-inducedalkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

Toxicity: Standard Handling (A)

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in PBS containing at least 0.1% human or bovine serum albumin.

Pinzone, J.J., et al. 2009. Blood113, 517.
Li, J., et al. 2006. Bone36, 754.
Morvan, F., et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C., 2006. Oncogene25, 7469.
Verani, R., et al. 2006. J. Neurochem.101, 242.
Cappuccio, I., et al. 2005. J. Neurosci.25, 2647.
Kemp, C., et al. 2005. Dev. Dyn.233, 1064.
Adamska, M., et al. 2004. Dev. Biol.272, 134.
Bullock, C.M., et al. 2004. Mol. Pharmacol.65, 582.
Mao, B., et al. 2002. Nature417, 664.
Mao, B., et al. 2001. Nature411, 321.
Glinka, A., et al. 1998. Nature391, 357.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 3