Colorwheel^® Antibody-Ready PerCP Dye

200

ColorWheel^®

lyophilized

flow cytometry: suitable

λ_ex 477 nm; λ_em 678 nm

ambient

2-8℃

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

PerCP (Peridinin-chlorophyll-protein complex) is a water-soluble dye that is isolated from a Dinophyceae sp., a photosynthetic dinoflagellate. It exhibits an excitation max at 477 nm and an emission max at 678 nm and has an extinction coefficient of 350,000 cm^-1 M^-1. PerCP is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS) and can be used for multicolor analysis with FITC, phycoerythrin, and other fluorochromes. Due to its photobleaching characteristics, PerCP conjugates may not be used on flow cytometers with high-power lasers.

Quality Control Testing:

Flow Cytometry: Excitation: 477 nm, Emission: 678 nm

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

CWDS-PERCP-25UL Set ComponentsCWD-PERCP-25UL - ColorWheel^® Antibody-Ready PerCP DyeLyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.CWD-FINISH-25UL - ColorWheel^® Finishing BufferLyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.