您的位置：首页 > 产品中心 > β-Galactose Dehydrogenase
β-Galactose Dehydrogenase
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 1.1.1.48 ( BRENDA | IUBMB ) |
| General description【一般描述】 | D-galactose:NAD+ 1-oxidoreductase |
| Specificity【特异性】 | Galactose dehydrogenase will oxidize D-galactose [Km = 0.7 mM (30 ℃, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galactose [galactosamine], (relative rate = 0.02; pH 8.6). Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (Km = 0.24 mM; relative rate = 1.0) or NADP (Km = 2.3 mM; relative rate = 0.32). |
| Quality【质量】 | Contaminants: <0.01% ADH, <0.01% ?-galactosidase, <0.1% NADH oxidase, <0.5% LDH |
| Sequence【序列】 | Galactose dehydrogenase from Pseudomonas fluorescens (MW 64,000 D) is a dimer with subunits of equal size (subunit MW = 32,000 D). |
| Unit Definition【单位定义】 | One unit galactose dehydrogenase will oxidize 1 mol of β-D-galactose in one minute at +25 ℃ and pH 8.6. The QC assay produces 1 mol of NADH per mol of galactose oxidized. |
| Physical form【外形】 | Suspension in 3.2 M ammonium sulfate solution, 1 mM EDTA, pH approximately 6 |
| Other Notes【其他说明】 | 仅用于生命科学研究。不可用于诊断。 |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | Pseudomonas fluorescens |
| form【形式】 | suspension |
| Specific Activity | ~5 units/mg protein (At 25 ℃ with D-galactose as the substrate; standardized with BSA.) |
| packaging【包装】 | pkg of 1 mL (10104973001 [1 mg]) pkg of 1 mL (10104981001 [5 mg]) |
| manufacturer/tradename | Roche |
| optimum pH【最佳pH】 | 8.4 (0.1 M in Tris buffer) |
| pH range【pH范围】 | 4.6 - 9.2 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
