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Anti-WAPL Antibody, clone 1C7-B1
产品别名
Anti-WAPL Antibody, clone 1C7-B1
WAPL, Wings apart-like protein homolog, Friend of EBNA2 protein
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Wings apart-like protein homolog (also known as Friend of Epstein-Barr virus nuclear protein 2) is a nuclear protein encoded by the WAPL (WAPAL) gene in human. Wapl functions as a negative regulator of cohesin–chromosome association and facilitates the progression of mitosis both during interphase and prophase. In interphase, Wapl destabilizes cohesin–chromosome interaction and thereby reduces the extent of cohesin-mediated chromosome looping. In prophase, mitotic kinases phosphorylate cohesin and its regulators. Wapl then triggers the release of phosphorylated cohesin from sister chromatids, allowing sister chromosome decatenation by topoisomerase II (Topo II). (PMID 24156811). |
| Immunogen【免疫原】 | Recombinant protein corresponding to human WAPL. |
| Application【应用】 | Immunoprecipitation Analysis: 1.0 mL tissue culture supernantant of this antibody immunoprecipitated WAPL in HeLa cell lysate (Professor Haruhiko Siomi, Keio University School of Medicine). Research Sub Category Nuclear Receptors Research Category Epigenetics & Nuclear Function Anti-WAPL Antibody, clone 1C7-B1 is an antibody against WAPL for use in Western Blotting, Immunoprecipitation. |
| Quality【质量】 | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected WAPL in 10 µg of HeLa cell lysate. |
| Physical form【外形】 | Format: Purified Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Protein G Purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 1C7-B1, monoclonal |
| species reactivity | human |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG2aκ |
| NCBI accession no.【NCBI登记号】 | NP_055860 |
| UniProt accession no.【UniProt登记号】 | Q7Z5K2 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... WAPAL(23063) |
产品说明
| Target description【目标描述】 | ~175 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
