Anti-UDP-Galactose Transporter Antibody, clone 1H10.1

32160702

UDP-galactose translocator (UniProt P78381; also known as Solute carrier family 35 member A2, UDP-Gal-Tr, UDP-galactose transporter, UDP-galactose transporter, UGT) is encoded by the SLC35A2 (also known as CDG2M, UGALT, UGT, UGTL) gene (Gene ID 7355) in human. UGT is a multi-pass membrane protein that transports UDP-galactose from the cytosol into the Golgi vesicles where it serves as a glycosyl donor for the generation of glycans. Its short N-terminal region, composed of 35 amino-acid residues, is reported to be crucial for galactosylation of N-glycans. Mutations in SLC35A2 gene are known to reduce UDP-galactose transport that leads to galactose-deficient glycoproteins resulting in developmental delays, hyotonia, ocular anomalies, and brain malfunctions. Higher expression of UGT mRNA has been reported in human colon cancer. Ref.: Ng, BG., et al. (2013). Am. J. Human Gen. 92, 632-636.

The immunogen sequence is 100% present in spliced isoforms 1 (UGT2) and 3, while being only partially present in spliced isoforms 2 (UGT1), 4, and 5 reported by UniProt (P78381), reactivity toward these latter isoforms has not been confirmed.

KLH-conjugated linear peptide corresponding to human UDP-Gal transporter C-terminal end sequence.

Immunocytochemistry Analysis: Clone 1H10.1 hybridoma culture supernatant immunostained Golgi apparatus in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized A431 cells.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected UDP-Gal transporter in human colon, liver, lung, and placenta tissue sections.
Research Category
Apoptosis & Cancer
Detect UDP-galactose transporter (SLC35A2) using this mouse monoclonal Anti-UDP-Galactose Transporter, clone 1H10.1, Cat. No. MABC1205, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Evaluated by Western Blotting in human placenta tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UDP-Gal Transporter in 10 µg of human placenta tissue lysate.

Protein G purified.
Format: Purified
Purified mouse IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

100

mouse

purified antibody

primary antibodies

1H10.1, monoclonal

human

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG3κ

NP_005651

P78381

ambient

human ... SLC35A2(7355)

~50 kDa observed. 14.31/25.18 k.Da (isoform 1/3) calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1