Anti-Tie2 Mouse mAb (Ab33)

100

mouse

purified antibody

primary antibodies

Ab33, monoclonal

liquid

≤0.1% sodium azide as preservative

porcine, mouse, rat, human

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG1

wet ice

−20℃

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Protein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.
Recognizes the ~140-45 kDa Tie2 protein.
This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.

Human
a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag sequence

Frozen Sections (see comments)

Immunoblotting (0.2-1 µg/ml, chemiluminescence)

Immunoprecipitation (see comments)

Paraffin Sections (not recommended)

Toxicity: Standard Handling (A)

In 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.

Following initial thaw, aliquot and freeze (-20°C).

Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 8829.
Wong, A.L., et al. 1997. Circ. Res.81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer77, 51.
For frozen sections, tissues were treated with Triton^™-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

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10 - Combustible liquids

WGK 1