Anti-TET1 Antibody, clone 5D6

32160702

Methylcytosine dioxygenase TET1 (EC 1.14.11.n2; UniProt Q3URK3; also known as CXXC-type zinc finger protein 6, Ten-eleven translocation 1 gene protein homolog) is encoded by the Tet1 (also know as Cxxc6, Kiaa1676) gene (Gene ID 52463) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.

Clone 5D6 detected exogenously expressed TET1, but not TET2 or TET3, GST fusion protein (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).

His-tagged recombinant fragment from the region between DNA-binding and 2-oxoglutarate-binding domains of mouse TET1.
Epitope: Region between DNA-binding and 2-oxoglutarate-binding domains

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Anti-TET1 Antibody, clone 5D6 is an antibody against TET1 for use in Immunocytochemistry, Western Blotting, Immunoprecipitation, ELISA.
Western Blotting Analysis: A representative lot detected the endogenous TET1 in murine embryonic stem cell (mESC) lysate, as well as exogenously expressed TET1, but not TET2 or TET3, GST fusion in lysates from respective HEK293T transfectants (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous TET1 from murine embryonic stem cell (mESC) lysate. Subsequent LC-MS/MS analysis detected the presence of co-immunoprecipitated glycosyltransferase OGT (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Anaylsis: Clone 5D6 hybridoma culture supernatant was confirmed for its immunoreactivity against His-tagged murine TET1 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).

Evaluated by Immunocytochemistry in mouse ESCs.

Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected TET1 in mouse embryonic stem cells (ESCs).

Replaces: ABE1034

Protein G purified.
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Concentration: Please refer to lot specific datasheet.

100

rat

purified immunoglobulin

primary antibodies

5D6, monoclonal

mouse

ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG2aκ

NP_081660

Q3URK3

219.3 calculated.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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