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Anti-TAF10/TAFII30 Antibody, clone 6TA-2B11

产品编号:4154993
规格:ascites fluid, clone 6TA-2B11, from mouse
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4154993100 μL5350
产品别名

Anti-TAF10/TAFII30 Antibody, clone 6TA-2B11

Transcription initiation factor TFIID subunit 10, STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
Transcription initiation factor TFIID subunit 10 (UniProt Q12962; also known as STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit) is encoded by the TAF10 (also known as TAF2A, TAF2H, TAFII30) gene (Gene ID 6881) in human.Transcription initiation factor TFIID subunit 10 (UniProt Q12962; also known as STAF28, TAF(II)30, TAFII-30, TAFII30, Transcription initiation factor TFIID 30 kDa subunit) is encoded by the TAF10 (also known as TAF2A, TAF2H, TAFII30) gene (Gene ID 6881) in human. TAF10 (TAFII30) is a subunit of both the general transcription factor (GTF) TFIID and the coactivator SAGA (Spt-Ada-Gcn5-acetyltransferase) histone acetyltransferase (HAT) complexes involved in the initiation of RNA polymerase II (Pol II) transcription in eukaryotes. TFIID complex is composed of the TATA-binding protein (TBP) and thirteen TBP-associated factors (TAFs) that allow its recognition of not only TATA box-containing, but also TATA-less promoters for site-specific assembly of preinitiation complex (PIC). TAF10-knockout studies in mice indicate that TAF10 plays an important role during embryonic development and homeostasis in a tissue-dependent manner. TAF10 ablation is reported to result in severe anemia and lethality at embryonic stage E13.5 due to impaired erythropoiesis. In addition, TAF10 is shown to not only interact with the transcription factor GATA1, but also regulate its transcription as evidenced by its occupancy at GATA1 genomic locus in human fetal erythroid cells.
Specificity【特异性】
Clone 6TA-2B11 (a.k.a. 6TA 2B11) specifically immunostained wild-type, but not Taf10-/-, mouse embryonic blastocysts (Mohan, W.S. Jr, et al. (2003). Mol .Cell. Biol. 23(12):4307-4318).
Immunogen【免疫原】
Epitope: Internal (N-terminal half).
Recombinant human TAF10/TAFII30.
Application【应用】
Research Sub Category
Transcription Factors
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected TAF10/TAFII30 in A431 and HeLa cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TAF10/TAFII30 in TAFIID complex isolated from HeLa nuclear extract (Courtesy of Dr. Mustapha OULAD-ABDELGHANI, IGBMC, France).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TAF10 together with associated GATA1 from murine erythroleukemia (MEL) cell nuclear extracts (Papadopoulos, P., et al. (2015). Mol. Cell. Biol. 35(12):2103-2118).
Western Blotting Analysis: A representative lot detected TAF10 in E12.5 mouse fetal liver nuclear extracts and murine erythroleukemia (MEL) cell nuclear extracts, as well as in GATA1 immunoprecipitate from MEL cell and fetal liver nuclear extracts (Papadopoulos, P., et al. (2015). Mol. Cell. Biol. 35(12):2103-2118).
Immunocytochemistry Analysis: A representative lot detected TAF10 immunoreactivity in trophoblasts from 3-day cultured wild-type, but not Taf10-/-mouse embryonic blastocysts isolated at 3.5 dpc (days post-coitum) by immunofluorescent staining of 1% paraformaldehyde-fixed, 0.1% Tween 20-permeabilized blastocysts (Mohan, W.S. Jr, et al. (2003). Mol .Cell. Biol. 23(12):4307-4318).
Research Category
Epigenetics & Nuclear Function
Anti-TAF10Antibody, clone 6TA-2B11 is an antibody against TAFII30 for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.
Quality【质量】
Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected TAF10/TAFII30 in 10 µg of A431 cell lysate.
Physical form【外形】
Unpurified
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Other Notes【其他说明】
Concentration: Please refer to lot specific datasheet.
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
ascites fluid
antibody product type
primary antibodies
clone【克隆】
6TA-2B11, monoclonal
species reactivity
human, mouse
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype【同位素/亚型】
IgG1κ
NCBI accession no.【NCBI登记号】
NP_006275
UniProt accession no.【UniProt登记号】
Q12962
shipped in【运输】
dry ice
Gene Information
human ... TAF10(6881)
产品说明
Target description【目标描述】
~30 kDa observed. 21.71 kDa calculated.
Storage and Stability【储存及稳定性】
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 1
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