Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG)

32160702

NA.43

T7 (MASMTGGQQMG). Rabbits were immunized with T7 (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Epitope: T7 (MASMTGGQQMG)
MASMTGGQQMG (T7) conjugated to KLH

Research Sub Category
Epitope Tags
Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG) detects level of T7 Epitope Tag & has been published & validated for use in IP, WB & IC.
Western blot: 1:1,000-1:10,000 colorimetric; 1:1,000-1:30,000 ECL.

Immunocytochemistry: 1:100-1:400

ELISA: 1:100-1:500 for coating; 1:1,000-1:30,000 for primary.

Immunoprecipitation: 2-5μg/mL in 300-500μL of cell lysate (150-300μg/mL total protein). Lysis buffer 0.5% triton X-100 with proteinase inhibitors. Use highquality protein A beads or anti-rabbit conjugated beads for best results. Run bead-only (no T7 antibody) controls to mark bands related to protein A breakdown products.



Optimal working dilutions must be determined by the end user.
Research Category
Epitope Tags & General Use

Affinity purified immunoglobulin. Liquid in PBS, pH 7.2 with 0.1% sodium azide.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity purified immunoglobulin

polyclonal

affinity chromatography

all

Chemicon^®

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

wet ice

Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2