Anti-SAP-1 Antibody, clone 123

32160702

Receptor type protein tyrosine phosphatase sap-1 (UniProt: B6ZDS3; also known as Stomach-cancer associated protein tyrosine phosphatase 1, SAP-1) is encoded by Ptprh (also known as Sap1) gene (Gene ID: 545902) in murine species. SAP-1 is a receptor-type (R3) protein tyrosine phosphatase that is expressed predominantly in the gastrointestinal tract, most prominently in the microvilli of the brush border in gastrointestinal epithelial cells. SAP-1 has a single catalytic domain in its cytoplasmic region and eight fibronectin type III-like domains in its extracellular region. It is synthesized with a 27 amino acid signal peptide, which is subsequently cleaved. SAP-1 is reported to play a significant role in the regulation of intestinal tumorigenesis and its expression is markedly increased in colon and pancreatic cancers. Ablation of SAP-1 in IL-10-deficient mice display greater severity of colitis in association with up-regulation of mRNAs for various cytokines and chemokines in the colon. The cytoplasmic region of SAP-1 is shown to bind directly to Lck. Over-expression of SAP-1 results in down-regulation of Lck activity that leads to inhibition of T cell receptor-mediated T cell function. (Ref.: Murata, Y et al. (2015). Proc. Natl. Acad. Sci. USA. 112(31):E4264-71).

Clone 123 recognizes SAP-1 in HEK293A cells transfected with murine SAP-1. It targets an epitope in the extracellular domain.

Recombinant fusion protein comprising the extracellular region of mouse SAP-1 (amino acids 1-604) and the Fc region of human IgG.

Epitope: extracellular domain

Detect SAP1 using this rat monoclonal Anti-SAP-1 Antibody, clone 123, Cat. No. MABC1592. It has been tested in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western blotting.
Western Blotting Analysis: A representative lot detected SAP-1 in HEK293A cells transfected with murine SAP-1 versus mock control. (Courtesy of Yoji Murata, Ph.D. and Takashi Matozaki, M.D., Ph.D. Kobe University Graduate School of Medicine, Japan).

Immunofluorescence Analysis: A representative lot detected SAP-1 in Immunofluorescence applications (Sadakata, H., et. al. (2009). Genes Cells. 14(3):295-308; Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71).

Immunofluorescence Analysis: A representative lot detected SAP-1 in ileum from 8-week old WT mouse (Courtesy of Yoji Murata, Ph.D. and Takashi Matozaki, M.D., Ph.D. Kobe University Graduate School of Medicine, Japan).

Immunoprecipitation Analysis: A representative lot detected SAP-1 in Immunoprecipitation applications (Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71).

Immunohistochemistry Analysis: A representative lot detected SAP-1 in Immunohistochemistry applications (Sadakata, H., et. al. (2009). Genes Cells. 14(3):295-308; Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71).

Western Blotting Analysis: A representative lot detected SAP-1 in Western Blotting applications (Sadakata, H., et. al. (2009). Genes Cells. 14(3):295-308; Murata, Y., et. al. (2015). Proc Natl Acad Sci USA. 112(31):E4264-71).

Research Category
Apoptosis & Cancer

Evaluated by Western Blotting in HEK293A cells transfected with murine SAP-1.

Western Blotting Analysis: 1 µg/mL of this antibody detected SAP-1 in 10 µg of lysate from HEK293A cells transfected with murine SAP-1.

Protein G purified
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Concentration: Please refer to lot specific datasheet.

100

rat

purified immunoglobulin

primary antibodies

123, monoclonal

mouse

human

immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

B6ZDS3

ambient

~180 kDa observed; 106.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1