Anti-P2X4 Receptor Antibody

32160702

NA.41

Recognizes a full-length P2X4 purinergic receptor protein and not with other known proteins tested so far.

SPECIES REACTIVITIES: The immunogen sequence is identical in mouse. Other species have not been tested.

A purified peptide of rat P2X4 protein (amino acids 370-388) (Accession P51577).

Anti-P2X4 Receptor Antibody detects level of P2X4 Receptor & has been published & validated for use in WB.
Research Category
Neuroscience
Western blot: 1-1.5 μg/mL (1:200:1:300) using ECL

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Research Sub Category
Neurotransmitters & Receptors

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

rat

Chemicon^®

western blot: suitable

NM_002560.2

Q99571

wet ice

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

H412

P273 - P501

Aquatic Chronic 3

11 - Combustible Solids

WGK 3