Anti-Integrin α5 Antibody, CT, Intracellular

32160702

NA.41

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.

Antibody recognizes integrin alpha5. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. No cross-reactivity to alpha1, alpha2, alpha3, alpha4, alpha6 or alphaV. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus, intracellular.
Reactivity with other species has not been determined.

Synthetic peptide derived from the C-terminal sequence (cytoplasmic domain) of the human alpha5 integrin subunit.

Anti-Integrin α5 Antibody, C-terminus, Intracellular detects level of Integrin α5 & has been published & validated for use in ELISA, IC, IF, IH, IP, RIA & WB.
Western Blot Analysis:
1:1000 dilution of a previous lot was used.

ELISA:
1:500-1:2000 dilution of a previous lot was used in ELISA.

Immunoprecipitation:
Recommended use of 5 μL of antibody for 5x106 cells.

Immunohistochemistry:
1:1000 dilution of a previous lot was used in tissue staining; suggested for use on acetone fixed tissue only.

Immunocytochemistry:
1:1000 of a previous lot was used in immunocytochemistry.

Optimal working dilutions must be determined by the end user.

Routinely evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected integrin alpha 5 on 10 μg of HUVEC lysates.

Rabbit polyclonal antiserum in buffer containing 0.05% sodium azide.
Unpurified

Control
Mouse 3T3 fibroblasts Skin (Basement Membrane).

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

serum

primary antibodies

polyclonal

human, rat, hamster, chicken, mouse

Chemicon^®

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

NP_002196

P08648

dry ice

human ... ITGA5(3678)

114 kDa

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1