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Anti-Hsp70 Mouse mAb (C92F3A-5)

产品编号:4154484
规格:liquid, clone C92F3A-5, Calbiochem®
包装规格:50 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
415448450 μG5850
产品别名

Anti-Hsp70 Mouse mAb (C92F3A-5)

Anti-Heat Shock Protein 70

产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
C92F3A-5, monoclonal
form【形式】
liquid
contains【包含】
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
avoid repeated freeze/thaw cycles
isotype【同位素/亚型】
IgG1
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
packaging【包装】
50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
基本信息
General description【一般描述】
Mouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.
Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections).
Recognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.
Immunogen【免疫原】
Hsp70 from HeLa cells
Human
Application【应用】
ELISA (see comments)

Flow Cytometry (see comments)

Frozen Sections (5 µg/ml)

Immunoblotting (1 µg/ml)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (see comments)

Paraffin Sections (5 µg/ml)
Warning【警告】
Toxicity: Standard Handling (A)
Physical form【外形】
In PBS, 50% glycerol, pH 7.2.
Reconstitution【重悬】
Following iniital thaw, aliquot and freeze (-20°C).
Analysis Note【分析说明】
Positive Control
L929 cells, Human colon cancer tissue
Other Notes【其他说明】
Does not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Hang, H., and Fox, M.H. 1995. Cytometry19, 119.
Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589.
Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724.
Gower, D.J., et al. 1989. J. Neurosurg.70, 605.
Milarski, K., et al. 1989.J. Cell Biol.108, 413.
Vass, K., et al. 1988. Acta Neuropathologica77, 128.
Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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