Anti-GABA(A) Receptor α2 Antibody, clone N399/19

32160702

NA.41

Gamma-aminobutyric acid receptor subunit alpha-2 (UniProt P23576; also known as GABA(A) receptor subunit alpha-2, Gamma-aminobutyric acid receptor A2 subunit) is encoded by the Gabra2 (also known as Gabra-2) gene (Gene ID 289606) in rat species. The GABAA receptor (GABAAR) is an ionotropic receptor and ligand-gated ion channel. Its endogenous ligand is the inhibitory neurotransmitter γ-aminobutyric acid (GABA) in the central nervous system (CNS). Upon activation, the GABAA receptor selectively conducts chloride ion through its pore, resulting in hyperpolarization of the neuron. The receptor is a pentameric complex composed of two alpha, two beta, and one gamma subunits arranged around a central pore. Each subunit contains four transmembrane domains with both the N- and C-terminus located extracellularly. In human and rat, there exist six types of alpha subunits (encoded by GABRA1-6), three types of beta subunits (encoded by GABRB1-3), and three types of gamma subunits (encoded by GABRG1-3). The alpha 2 subunit is intially produced with a signal peptide sequence (aa 1-28), which is then cleaved off to yield the mature subunit (aa 29-451) with a large N-terminal extracellular domain (a.a. 29-251), four transmembrane segments (a.a. 252-273, 279-300, 313-334, 420-441) and a large intracellular/cytoplasmic loop (a.a. 335-419) between the third and fourth transmembrane domain.

Clone N399/19 detects the GABA(A) receptor subunit alpha-2 band only in brain membrane extracts from wild-type rats and mice, but not from Gabra2-knockout mice. Clone N399/19 does not cross-react with GABA(A) receptor subunit alpha-1.

Epitope: Cytoplasmic domain.
Recombinant rat GABA(A) receptor α2 cytoplasmic fragment.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected GABA(A) receptor subunit alpha-2 in 10 µg rat brain tissue lysate.
Western Blotting Analysis: A representative lot detected the GABA(A) receptor subunit alpha-2 target band only in brain membrane extracts from wild-type rats and mice, but not from Gabra2-knockout mice.
Immunohistochemisry Analysis: A representative lot detected GABA(A) receptor subunit alpha-2 immunoreactive regions, includinghippocampus, in rat whole brain sections (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Detect GABA(A) Receptor α2 using this Anti-GABA(A) Receptor α2 Antibody, clone N399/19 validated for use in Western Blotting, Immunohistochemistry.

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GABA(A) receptor subunit alpha-2 in 10 µg human brain tissue lysate.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

N399/19, monoclonal

human, mouse, rat

immunohistochemistry: suitable
western blot: suitable

IgG1κ

P23576

wet ice

~57 kDa observed. Target band appears larger than the calculated molecular weight of 47.94 kDa due to glycosylation.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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