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Anti-Giα-3-Subunit, C-Terminal (345-354) Rabbit pAb
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | liquid |
| does not contain【不包含】 | preservative |
| species reactivity (predicted by homology) | mammals |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
| isotype【同位素/亚型】 | IgG |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −70℃ |
| packaging【包装】 | 50 μL in Glass bottle |
基本信息
| General description【一般描述】 | Anti-Giα-3-Subunit, C-Terminal (345-354), rabbit polyclonal, recognizes Giα-3 subunit. Does not cross-react with Gsα & Goα subunits. Validated for use in WB & IP. Immunoaffinity purified rabbit polyclonal antibody. Recognizes the Giα-3 subunit protein. Recognizes Giα-3 subunit. Does not cross-react with Gsα and Goα, but partially cross-reacts with Giα1-, G1α2-subunits. |
| Immunogen【免疫原】 | a synthetic peptide (CKNNLKECGLY) corresponding to amino acids at the C-terminus of mammalian Giα-3, conjugated to KLH |
| Application【应用】 | Immunoblotting (1:1000) Immunoprecipitation (see comments) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5. |
| Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
| Other Notes【其他说明】 | Kumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537. Raymond, J.R., et al. 1993. Biochemistry32, 11064. Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215. Jones, D.T., and Reed, R.R. 1987. J. Biol. Chem.262, 14,241. The specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. The original titer of the serum was 1:10,000. Suitable for immunoblotting and immunoprecipitation. Does not cross-react with Gsα and Goα. Antibody partially cross-reacts with Giα-1 and GIα-2. Variables associated with assay conditions will dictate the proper working dilution. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
