Anti-DEDAF Antibody

32160702

NA.41

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. DEDAF (DED Associated Factor) is expressed in multiple tissues and cell lines. DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and augments the formation of CD95/FADD/Caspase-8 complexes at the cell membrane. DEDAF also interacts with DEDD (DED-containing DNA binding) protein in the nucleus, which indicates its involvement in the regulation of both cytoplasmic and nuclear events of apoptosis.

Rabbit anti-DEDAF polyclonal antibody was raised against a synthetic peptide corresponding to amino acids 215 to 228 of human-DEDAF. This sequence is identical to the mouse sequence.

Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
Western blot: 0.5-1 μg/mL.

A549 or HepG2 cell lysate can be used as a positive control and a 32 kDa band should be detected.

Optimal working dilutions must be determined by end user.
This Anti-DEDAF Antibody is validated for use in WB for the detection of DEDAF.

Purified IgG by immunoaffinity chromatography. Liquid in PBS containing 0.02% sodium azide.
Format: Purified

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity purified immunoglobulin

primary antibodies

polyclonal

mouse, human, rat

Chemicon^®

western blot: suitable

NM_012234.4

Q8N488

wet ice

human ... RYBP(23429)

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2