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Anti-DDX4 (Vasa) Antibody
产品编号: | 4154311 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-DDX4 (Vasa) Antibody
DEAD (Asp-Glu-Ala-Asp) box polypeptide 4, probable ATP-dependent RNA helicase DDX4, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4, Vasa homolog, DEAD box protein 4
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | DDX4 (Vasa or MVH) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. DDX4 (Vasa) proteins are mostly localized in testicular germ cells in the cytoplasm of spermatocytes. DDX4 (Vasa) is a high-copy extragenic suppressor gene. DDX4 (Vasa) is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility. |
Specificity【特异性】 | This antibody recognizes DDX4 (Vasa) at the C-terminus. |
Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to the C-terminus of mouse DDX4 (Vasa). Epitope: C-terminus |
Application【应用】 | Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected DDX4 (Vasa) in normal human testis tissue. Research Sub Category Pluripotent & Early Differentiation RNA Metabolism & Binding Proteins Anti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P). Research Category Stem Cell Research |
Quality【质量】 | Evaluated by Western Blot in mouse testis tissue lysate. Western Blot Analysis: A 1:500 dilution of this antibody detected DDX4 (Vasa) in 10 µg of mouse testis tissue lysate. Western Blot performed without Tween. |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. Affinity purified |
Analysis Note【分析说明】 | Control Mouse testis tissue lysate |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | rat, mouse, human |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_034159 |
UniProt accession no.【UniProt登记号】 | Q61496 |
shipped in【运输】 | wet ice |
Gene Information | human ... DDX4(54514) |
产品说明
Target description【目标描述】 | ~76 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |