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Anti-Cytochrome P450 Enzyme CYP3A4 Antibody
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
Specificity【特异性】 | The rabbit polyclonal serum detects CYP3A4 in humans and rats; by sequence homology of the immunogen used, cross reactivity to CYP3A7, 3A6v4;3A2v1, 3A8, 3A9 may be expected but is untested at this time. |
Immunogen【免疫原】 | A synthetic peptide containing the C-terminal sequence of TVSGA from human CYP3A4. The peptide was coupled to KLH. |
Application【应用】 | Research Category Metabolism Research Sub Category Enzymes & Biochemistry Western blot: 1:1000-1:2000: Using microsomal membrane fractions, detects a band at ~54kDa; other bands may be present as well. Antibody has been successfully used to detect CYP3A4 on LICOR fluorescent westerns as well. Immunohistochemistry: 10% formalin fixed, paraffin embedded tissues 1:200-1:400, microwave-citric acid antigen recovery is suggested. Optimal working dilutions must be determined by end user. Anti-Cytochrome P450 Enzyme CYP3A4 Antibody detects level of Cytochrome P450 Enzyme CYP3A4 & has been published & validated for use in IH & WB. |
Physical form【外形】 | Unpurified Rabbit antiSerum. Liquid containing no preservative. |
Analysis Note【分析说明】 | Control Liver tissue |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | serum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | human, pig |
species reactivity (predicted by homology) | rat, mammals |
manufacturer/tradename | Chemicon® |
technique(s) | immunohistochemistry: suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NM_017460.3 |
UniProt accession no.【UniProt登记号】 | P08684 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | 57 kDa |
Storage and Stability【储存及稳定性】 | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
WGK | WGK 1 |