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Anti-Calreticulin Mouse mAb (FMC75)

产品编号:4154211
规格:liquid, clone FMC75, Calbiochem®
包装规格:50 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
415421150 μG6020
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
FMC75, monoclonal
form【形式】
liquid
does not contain【不包含】
preservative
species reactivity
human, monkey
should not react with
hamster, bovine, canine, guinea pig, E. coli, mouse, rat, salmon
manufacturer/tradename
Calbiochem®
storage condition【储存条件】
OK to freeze
avoid repeated freeze/thaw cycles
isotype【同位素/亚型】
IgG1
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
packaging【包装】
50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
基本信息
General description【一般描述】
Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.
Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.
Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.
Immunogen【免疫原】
a recombinant protein consisting of human calreticulin fused to maltose binding protein
Human
Application【应用】
Enzyme Immunoassay (0.1 µg/ml)

Immunoblotting (0.5 µg/ml, colorimetric; see application references)

Immunocytochemistry (see application references)

Immunohistochemistry (see application references)

Flow Cytometry (see application references)
Warning【警告】
Toxicity: Standard Handling (A)
Physical form【外形】
In PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.
Reconstitution【重悬】
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note【分析说明】
Positive Control
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
Other Notes【其他说明】
Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information【法律信息】
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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