Anti-Beta III Tubulin Antibody

32160702

NA.41

Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division, and intracellular trafficking. There are at least three types of tubulins: alpha, beta, and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. Class III β-tubulin‎ is a microtubule element expressed exclusively in neurons, and is a popular marker specific for neurons in tissue.

Recognizes Beta III Tubulin

Epitope: (Similar to TuJ1 antigen)
Synthetic peptides from human/rat Beta III Tubulin.

Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism
Anti-Beta III Tubulin Antibody detects level of Beta III Tubulin & has been published & validated for use in IC, IH & WB.
Immunocytochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunocytochemistry using 2% paraformaldehyde fixed cells.

Immunohistochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunohistochemistry using 2% paraformaldehyde fixed tissues.

Optimal working dilutions must be determined by the end user

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot:
1:1000 dilution of this lot detected TUBULIN BETA III on 10 μg of mouse brain lysates.

Replaces: 04-1049

ImmunoAffinity Purified
Affinity purified polyclonal chicken IgY liquid in PBS, pH 7.2 with 0.02% sodium azide.
Four different antibodies were combined to make this product

Control
Brain tissue, Neuronal culture

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

chicken

100

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

mouse, rat, human

Chemicon^®

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgY

NM_006086.2

Q13509

wet ice

55 kDa

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
It is recommended that the antibody be stored in the dark. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2