Anti-Atg13 Antibody, clone 2H4.2

32160702

NA.41

The autophagy-related protein 13 (Atg13) plays an important role in the formation of autophagosomes. Autophagosomes are formed in response to nutrient deprivation and function as the transport vesicles for organelles, proteins, and protein complexes targeted for lysosomes that digest these cargos to produce energy and nutrients. Atg13 is activated by the mTOR pathway and forms a complex with the FIP200 protein. This complex is involved in enhancing the activity of the ULK1 kinase which is required for the formation of autophagosomes. Atg13/FIP200 faciliates the localization of ULK1 to pre-autophagosomes, and subsequently stabilizes ULK1. Autophagy is an important process in development, growth, and cell differentiation, and disruption of this process may contribute to cancer, aging, and neurodegenerative diseases.

GST-tagged recombinant protein corresponding to human Atg13.

Western Blot Analysis: A representative lot detected Atg13 in HeLa cell lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Atg13 in normal human substancia nigra tissues.
Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
This Anti-Atg13 Antibody, clone 2H4.2 is validated for use in Western Blotting, IHC for the detection of Atg13.

Evaluated by Western Blot in campthothecin treated HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Atg13 in 10 µg of campthothecin treated HeLa cell lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
Campthothecin treated HeLa cell lysate.

100

mouse

purified immunoglobulin

primary antibodies

2H4.2, monoclonal

human

immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_001136145

O75143

wet ice

~60 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1