Anti-Aquaporin 1 Antibody

32160702

NA.41

Aquaporin 1 is an integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

KLH-conjugated linear peptide corresponding to the topological domain of rat Aquaporin 1.
Epitope: Topological domain

Western Blotting: 1-10 μg/mL using Chemiluminescence technique. Immunoblots reveal two bands, a lower 28 kDa band corresponding to unglycosylated form and an upper high molecular 35-60 kDa glycosylated component (Denker et al. 1988; Smith & Agre 1991) We recommend the use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Aquaporin 1 in human kidney and mouse kidney tissue.


Research Category
Neuroscience
Anti-Aquaporin 1 Antibody detects level of Aquaporin 1 & has been published & validated for use in ELISA, WB, IH.
Research Sub Category
Developmental Signaling

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Aquaporin 1 in 10 µg of human kidney tissue lysate.

Replaces: AB3065

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
ImmunoAffinity Purified

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

rat, mouse, human

primate (based on 100% sequence homology), sheep (based on 100% sequence homology), bovine (based on 100% sequence homology)

Chemicon^®

1.0 mg/mL

immunohistochemistry: suitable
western blot: suitable

NP_036910

P29975

wet ice

human ... AQP1(358)

28 kDa

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1