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Anti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4
产品别名
Anti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4
Apolipoprotein E, Apo-E, ApoE4, Apolipoprotein E4
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | Apolipoprotein is a polymorphic plasma protein that exists as three isoforms: ApoE4, ApoE3, and ApoE2, which differ in the combination of cysteine and arginine residues located at position 122 and 158. The 35 kDa ApoE4 isoform contains arginine at both locations, and is produced in the liver and brain, although it is widely expressed in other tissues, such as the lung, spleen, and ovary. Like other isoforms, ApoE4 is a ligand for low density lipoprotein receptors (LDL) and plays a critical role in the transport and metabolism of lipids. In addition to its role in maintaining cardiovascular function, ApoE4 is also critically involved in neuronal plasticity, synaptogenesis, aging, and neurodegenerative disorders. Mice deficient in ApoE4 show age-dependent abnormalities in synaptic structure and impaired long term potentiation. Additionally, ApoE4 has a well-documented role in the pathology of Alzheimer’s disease. |
Specificity【特异性】 | This antibody recognizes ApoE4 but not ApoE3 or ApoE2. |
Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to human ApoE4. |
Application【应用】 | Immunohistochemistry Analysis: A 1:2,000-4,000 dilution from a representative lot detected ApoE4 in human Alzheimer′s diseased brain and human cerebellum tissues. Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect ApoE4 in CHO cells secreting ApoE4. (Image courtesy of Dr. Egon Ogris, Medical University of Vienna) Research Category Neuroscience This Anti--Apolipoprotein E4 antibody is validated for use in IP, WB, IH for the detection of ApoE4. Research Sub Category Developmental Neuroscience |
Quality【质量】 | Evaluated by Western Blot in recombinant ApoE4 protein. Western Blot Analysis: A 1:100,000 dilution detected ApoE4 in 10 µg of recombinant ApoE4 protein. |
Physical form【外形】 | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Protein G Purified |
Analysis Note【分析说明】 | Control Recombinant Apolipoprotein E protein |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | 4E4, monoclonal |
species reactivity | human |
technique(s) | immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG1κ |
NCBI accession no.【NCBI登记号】 | NP_000032 |
UniProt accession no.【UniProt登记号】 | P02649 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~35 kDa observed |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |