Anti-Aggrecan Antibody, clone 6B4

32160702

NA.41

Aggrecan (CSPG1, CSPCP, or CSPGCP) is a chondroitin-sulfate proteoglycan belonging to the family of letican proteins. Aggrecan is a large globar structure containing multiple domains including carbohydrate-recognition domains; complement binding protein domains; immunoglobulin domains; proteoglycan tandem repeats; and in some cases, EGF-like domains. It can be degraded by aggrecanase. The N-terminus of aggrecan binds ECM hyaluronan in the presence of the linking protein, and forms complex aggregates, whereas the C-terminus binds a number of other ECM proteins including tenascins, glycolipids, and fibulins. Aggrecan is a major component of cartilage tissue and it enables this tissue to withstand compression by maintaining the viso-elastic properties of cartilage. Aggrecan may also facilitate neural maturation and plasticity by contributing to the formation of perineuronal nets in the developing CNS.

Ovalbumin-conjugated linear peptide corresponding to human Aggrecan.

Research Category
Cell Structure
Anti-Aggrecan Antibody, clone 6B4 is an antibody against Aggrecan for use in Western Blotting & IHC.
Immunofluorescent Analysis: A representative lot was used by an independent laboratory in human femoral chondrocytes (Williams, R., et al. (2010). PLoS One. 5(10):e13246.).

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in human chartilage tissue (Williams, R., et al. (2010). PLoS One. 5(10):e13246.).
Research Sub Category
ECM Proteins

Evaluated by Western Blot in A1D1 purified protein from bovine tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Aggrecan in 10 µg of purified protein from bovine tissue lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
A1D1 purified protein from bovine tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

6B4, monoclonal

bovine

human (based on 100% sequence homology)

immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_001126

P16112

wet ice

human ... ACAN(176)

~60 kDa observed. Uniprot describes three isoforms at ~250 kDa, ~246 kDa, and ~239 kDa Aggrecan is degraded by aggrecanase into a ~60 kDa protein and other higher molecular weight products (Lemons, M. L. et al. (2001). J Neurosci. 21(13):4772-4781. Forsang, A. J. et al. (1995). Biochem J. 310(Pt 1):337-343.).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1