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Anti-5-Methylcytosine Mouse mAb (162 33 D3)
产品别名
Anti-5-Methylcytosine Mouse mAb (162 33 D3)
Anti-5-mc, Anti-5-MeCyd
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 162 33 D3, monoclonal |
| form【形式】 | liquid |
| does not contain【不包含】 | preservative |
| species reactivity (predicted by homology) | all |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
| isotype【同位素/亚型】 | IgG1 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 50 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | Immunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine. This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine. Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells. Specific • Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications • Flow Cytometry • Frozen Sections • Immunoblotting • Immunofluorescence • Paraffin Sections Reliable • Generates reproducible results. • Clone 162 33 D3 has been used in over 50 publications. |
| Immunogen【免疫原】 | 5-methylcytosine conjugated to ovalbumin |
| Application【应用】 |   ELISA (0.1 g/ml) Flow Cytometry (1 g/ml) Frozen Sections (5-10 g/ml) Immunoblotting (5 g/ml) Immunofluorescence (1 g/ml) Paraffin Sections (5-10 g/ml) Radioimmunoassay (0.1 g/ml) Southwestern Blot (5 g/ml) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | In PBS, pH 7.4. |
| Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
| Analysis Note【分析说明】 | Positive Control NIH3T3 cells |
| Other Notes【其他说明】 | Clone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems. Ehrlich, M. 2002. Oncogene21, 5400. (Review) Widschwendter, M. and Jones, P.A. 2002. Oncogene21, 5462. (Review) Barton, S.C., et al. 2001. Hum. Mol. Genet.10, 2983. Piyathilake, C.J., et al. 2001. Hum. Pathol.32, 856. Taddei, A., et al. 2001. Nat. Cell Biol.3, 114. Piyathilake, C.J., et al. 2000. Biotech. Histochem.75, 251. De Capoa, A., et al. 1999. FASEB J.13, 89. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
