Anti-Fas Ligand Antibody

32160702

NA.41

Human FAS Ligand (FASL). The 13-mer human FASL sequence used for antibody production is 92% (12/13 AA) homologous with rat and mouse FASL. Based upon the sequence homology data, anti-human FASL will likely cross react with the mouse/rat FASL.

A 13 AA peptide sequence mapping near the C-terminus of human FAS ligand (Mita et al. 1994). This peptide is predicted to be extracellular. The peptide was synthesized with C-terminal Cysteine, coupled to KLH.

Western Blotting: 1:1,000-1:5,000 Rat, mouse and human FASL have 278, 279, and 281 AA residues, respectively (calculated MW 32kDa). Western blot of the oocyte has detected a specific 31kDa band (Hakuno et al. 1996). A 40 kDa FASL has been detected in mouse T cells (Hahne 1995). An alternate detecting reagent, mFAS-Fc (Suda & Nagata 1994) also detected a cell surface protein of about 40 kDa.

ELISA: 0.5-1μg/mL

Optimal working dilutions must be determined by the end user.
Research Sub Category
Apoptosis - Additional
Anti-Fas Ligand Antibody detects level of Fas Ligand & has been published & validated for use in ELISA & WB.
Research Category
Apoptosis & Cancer

Peptide affinity purified immunoglobulin. Liquid in PBS, containing 0.1% BSA.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

mouse, human, rat

Chemicon^®

ELISA: suitable
western blot: suitable

NM_000043.3
NM_152871.1
NM_152872.1
NM_152873.1
NM_152874.1
NM_152875.1
NM_152876.1
NM_152877.1

P25445

dry ice

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2