Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)

100

mouse

purified antibody

primary antibodies

13/45/31/2, monoclonal

liquid

≤0.1% sodium azide as preservative

all

Calbiochem^®

do not freeze

IgG1

wet ice

2-8℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His•Tag sequence in native and recombinant proteins. Specifically recognizes an epitope of six consecutive His residues of both natural and recombinant sources.
Specifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.

Epitope: HHHHHH
a recombinant protein containing a His•Tag sequence

ELISA (0.1-0.4 g/ml)
Immunoblotting (1-4 g/ml)
Immunofluorescence (1-4 g/ml)
Immunoprecipitation (4 g/ml)

Toxicity: Standard Handling (A)

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Positive Control
Proteins containing a His•Tag sequence

Zentgraf, H., et al. 1995. Nucl. Acids. Res.23, 3347.
Gu, J., et al. 1994. BioTechniques17, 257.
Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994. J. Virol.68, 766.
Lu, T., et al. 1993. Anal. Biochem.213, 318.
Garner, J., et al. 1992. Cell69, 833.
Hochuli, E., et al. 1987. J. Chromatogr.411, 177.
Antibody should be titrated for optimal results in individual systems.

Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 1