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ABScript II One Step RT-qPCR Probe Kit
产品别名
ABScript II One Step RT-qPCR Probe Kit
One Step RT-qPCR master mix
Probe-based RT-qPCR
产品性质
| Quality Level【质量水平】 | 200 |
| usage【用途】 | sufficient for 100 reactions sufficient for 20 reactions sufficient for 500 reactions |
| feature【特点】 | dNTPs included hotstart |
| technique(s) | DNA amplification: suitable PCR: suitable RT-PCR: suitable RT-qPCR: suitable cDNA synthesis: suitable qPCR: suitable |
| input | purified RNA |
| compatibility【相容性】 | ABI 7000 ABI 7300 ABI 7500 ABI 7700 ABI 7900 ABI StepOne ABI StepOnePlus ABI ViiA 7 Bio-Rad CFX384 Bio-Rad CFX96 Bio-Rad iCycler Eppendorf® Mastercycler ep realplex2 s Eppendorf® Mastercycler ep realplex Qiagen Corbett Rotor-Gene 3000 Qiagen Corbett Rotor-Gene 6000 Qiagen Corbett Rotor-Gene Q QuantStudio™ Roche LightCycler 480 Strategene Mx3000P Strategene Mx3005P Strategene Mx4000 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
基本信息
| General description【一般描述】 | The ABScript II One Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with optimized components for use for reverse transcription and subsequent probe-based qPCR. The one-step format prevents contamination and allows for higher sensitivity. This kit is ideal for analyzing low input RNA samples. |
| Application【应用】 | For use in real-time RT-PCR (or RT-qPCR) applications, including detection of RNA in samples being analyzed. The ABScript II One Step RT-qPCR Probe Kit is a ready-to-use kit for reverse transcription and subsequent probe-based qPCR in a single tube. The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription of RNA. After reverse transcription, the hot-start version of Taq polymerase is activated at 95℃ and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5′-3′ exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. For Research Use Only. Application Note Additional Materials Required but Not Included:
Compatible Instruments: *50X ROX Reference Dye I Applied Biosystems 7000/7300/7700/7900, Applied Biosystems StepOne™/StepOnePlus™ **50X ROX Reference Dye II Applied Biosystems 7500/ViiA™7, QuantStudio, Stratagene Real-time PCR Systems, Rotor-gene® 3000 NO ROX Reference Dye Bio-Rad iCyclers/ CFX96™/ CFX384™, Roche® Light Cyclers, QIAGEN/Corbett Systems, Eppendorf Mastercyclers®. |
| Features and Benefits【特点和优势】 |
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| Components【组分】 | 2X One Step RT-qPCR Probe Buffer* One Step Probe HS Taq ABScript II RT Enzyme Mix 50X ROX Dye I * 50X ROX Dye II ** RNase-free ddH2O See datasheet for more information. Reaction Size Reaction size is based on a 50 uL reaction volume. The 2x One Step RT-qPCR Probe Buffer is supplied in the following volumes: 20RXN: 0.5 mL 100RXN: 2.5 mL 500RXN: 1.25 mL |
| Legal Information【法律信息】 | CFX384 is a trademark of Bio-Rad Laboratories, Inc. CFX96 is a trademark of Bio-Rad Laboratories, Inc. Eppendorf is a registered trademark of Eppendorf AG Mastercycler is a registered trademark of Eppendorf AG QuantStudio is a trademark of IROA Technologies LLC Roche is a registered trademark of Roche Molecular Systems, Inc. Rotor-Gene is a registered trademark of Qiagen GmbH StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries ViiA is a trademark of ThermoFisher |
安全信息
| Pictograms【象形图】 | GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H334 |
| Precautionary Statements | P261 - P284 - P501 |
| Hazard Classifications【危险分类】 | Resp. Sens. 1 |
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
