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Anti-v-Src (Ab-1) Mouse mAb (327)
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 327, monoclonal |
| form【形式】 | liquid |
| contains【包含】 | ≤0.1% sodium azide as preservative |
| species reactivity | human, mouse, rat |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | do not freeze |
| isotype【同位素/亚型】 | IgG1 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation. Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein. This Anti-v-Src (Ab-1) Mouse mAb (327) is validated for use in Immunoblotting, Immunoprecipitation, Frozen Sections, Immunofluorescence, Paraffin Sections for the detection of v-Src (Ab-1). |
| Immunogen【免疫原】 | purified v-Src Epitope: within the SH3 domain of v-Src and c-Src |
| Application【应用】 | Immunoblotting (2.5 µg/ml, see application references) Immunoprecipitation (1 µg antibody or use Cat. No. OP07A; see application references) Frozen Sections (not recommended) Immunofluorescence (not recommended) Paraffin Sections (not recommended) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4. |
| Analysis Note【分析说明】 | Positive Control NIH/3T3 cells |
| Other Notes【其他说明】 | pp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active src kinase (see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems. Courtneidge, S.A. and Heber, A. 1987. Cell50, 1031. Cartwright, C.A., et al. 1985. Mol. Cell. Biol.5,, 2647. Courtneidge, S.A. 1985. EMBO J.4, 1471. Whitman, M., et al. 1985. Nature315, 239 Bolen, J.B., et al. 1984. Cell38, 767. Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci.77, 1311. Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci.76, 3159. Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci.75, 2021. Collett, M.S., et al. 1978. Cell15, 1363. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
