YSK4 (MAP3K19), Active

expressed in baculovirus infected Sf9 cells

70-95% (SDS-PAGE)

frozen liquid

30-45 nmol/min·mg (radiometric)

~83 kDa

0.05 μg/μL

NM_025052

dry ice

−70℃

human ... YSK4(80122)

Useful for kinase assay and Western blot.

Recombinant human YSK4 (MAP3K19) (941-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. YSK4 (MAP3K19, RCK) belongs to STE Ser/Thr protein kinase family and STE20 subfamily. The YSK4 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat ⁽¹⁾. The important paralog is MAP3K1 gene. The RCK ligand-binding domains form an octameric gating ring ⁽²⁾ in the large-conductance Ca²⁺-activated K⁺ channels ⁽³⁾. The YSK4 kinase function has not been made clearly yet.

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

10 - Combustible liquids

WGK 1