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PARP3 Active human
产品编号: | 4139541 |
规格: | recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE) |
包装规格: | 10 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
PARP3 Active human
ADPRTL2
IRT1
Poly (ADP-ribose) Polymerase 3
ADPRT3
NAD(+) ADP-ribosyltransferase 3
产品性质
biological source【生物来源】 | human |
recombinant【重组】 | expressed in baculovirus infected insect cells |
Assay【测定】 | ≥80% (SDS-PAGE) |
form【形式】 | aqueous solution |
mol wt【分子量】 | 87 kDa |
packaging【包装】 | pkg of 10 μg |
concentration【浓度】 | >0.02 mg/mL |
NCBI accession no.【NCBI登记号】 | NM_001003931 |
UniProt accession no.【UniProt登记号】 | Q9Y6F1 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
Gene Information | human ... PARP3(10039) |
基本信息
General description【一般描述】 | Human PARP3 (GenBank Accession No. NM_001003931), full length with N-terminal GST tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
Application【应用】 | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Unit Definition【单位定义】 | One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form. |
Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT. |
Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |