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PARP1 Active human
| 产品编号： | 4139538 |
| 规格： | recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE) |
| 包装规格： | 20 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
PARP1 Active human
NAD(+) ADP-ribosyltransferase 1
ADPRT
poly (ADP-ribose) polymerase 1
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | Human PARP1 (GenBank Accession No. NM_001618), full length with N-terminal GST tag, MW = 140 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| Application【应用】 | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Unit Definition【单位定义】 | One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form. |
| Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT. |
| Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in baculovirus infected insect cells |
| Assay【测定】 | ≥80% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| mol wt【分子量】 | 140 kDa |
| packaging【包装】 | pkg of 10 and 20 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| concentration【浓度】 | >0.02 mg/mL |
| NCBI accession no.【NCBI登记号】 | NM_001618 |
| UniProt accession no.【UniProt登记号】 | P09874 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... PARP1(142) |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
