NEK5, active, GST tagged human

NA.32

NEK5 or NIMA (never in mitosis gene a)-related kinase 5 is a serine/threonine kinase that is involved in cell cycle regulation. NEK5 has been shown to interact with NEK6 and NEK5 are required for the ibo1/nek6 ectopic outgrowth phenotype in epidermal cells. NEK6 interacts and co-localizes with NEK5 in a transient expression assay. The ibo1-3 mutation markedly increases the interaction between NEK6 and NEK5. NEK6 forms heterodimers with NEK5 to regulate cortical microtubule organization possibly through the phosphorylation of β-tubulins. The NEK5 gene is conserved in chimpanzee, cow, mouse, rat, and chicken.

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

After opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles.

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

human

expressed in baculovirus infected Sf9 cells

PRECISIO^®

≥70% (SDS-PAGE)

buffered aqueous glycerol solution

80-120 nmol/min·mg

~84 kDa

NM_199289

cell analysis

dry ice

−70℃

human ... NEK5(341676)

10 - Combustible liquids

WGK 1