Glycolysis Assay Kit

100

colorimetric

diabetes; cancer; endocrinological disorders

−20℃

The kit is suitable for the direct determination of L-Lactate produced by glycolysis in cell media samples and for the screening of glycolysis inhibitors.

Glycolysis is one of the major metabolic pathways cells undergo to produce energy and results in the production of pyruvate. One of the eventual end products of this process is lactate dehydrogenase-mediated conversion of pyruvate to L-lactate via lactic acid fermentation. This enzymatic mechanism allows L-lactate to serve as an indicator of glycolysis.

The Glycolysis Assay Kit is based on measuring the production of L-Lactate from glycolysis in cells. L-Lactate that is secreted into the cell media is quantified using a coupled reaction involving the lactate dehydrogenase catalyzed oxidation of L-lactate that generates pyruvate and NADH, which subsequently reduces a formazan dye. The intensity of the reduced dye, measured at 565 nm, is directly proportional to the L-lactate concentration in the sample, which in turn is directly proportional to the glycolytic rate of the cells.

10 - Combustible liquids

WGK 2