Anti-TAP2 Antibody, clone 429.4

32160702

Antigen peptide transporter 2 (UniProt Q03519; also known as APT2, ATP-binding cassette sub-family B member 3, Peptide supply factor 2, Peptide transporter involved in antigen processing 2, Peptide transporter PSF2, Peptide transporter TAP2, PSF-2, Really interesting new gene 11 protein) is encoded by the TAP2 (also known as ABCB3, PSF2, RING11, Y1) gene (Gene ID 6891) in human. TAP2 (ABCB3) belongs to the subfamily B of ATP-binding cassette (ABC) transporters, members of which also include TAP1 (ABCB2) and multidrug resistance protein 1 (MDR1, ABCB1, P-gp). TAP is a heterodimeric peptide transporter composed of TAP1 and TAP2 that dimerize via their nucleotide-binding domain (NBD; a.a. 503-510) in a head-to-tail fashion. TAP preferentially mediates the transport of 8- to 16-a.a. peptides into the ER in a process powered by ATP-binding and hydrolysis. TAP1 and TAP2 harbor a specialized N-terminal transmembrane domain (TMD0) that is followed by the TAP half-transporter core module composed of one TMD and one NBD. TMD0 is composed of a 4-TM bundle that serves as the interaction platform for the assembly of the peptide-loading complex (PLC).

Clone 429.4 was subcloned from the original 429.3 hybridoma reported by van Endert, P.M., et al. (1994). Immunity. 1(6):491-500. The epitope lies within TAP2 ABC transporter domain. Clone 429 detected TAP2 in HeLa, but not in the ST-EMO B-lymphoblastoid cell line derived from a patient with homozygous TAP2 deletion (TAP2-/-) (Courtesy of Peter van Endert, Ph.D., Institut Necker Enfants Malades (INEM), Centre De Medecine Moleculaire, Paris, France).

Baculovirus-expressed recombinant human TAP2 C-terminal fragment (van Endert, P.M., et al. (1994). Immunity. 1(6):491-500).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TAP2 in U2OS human osteosarcoma cells.

Immunocytochemistry Analysis: A 1:400 dilution from a representative lot detected TAP2 in HeLa, but not in the ST-EMO B-lymphoblastoid cell line derived from a patient with homozygous TAP2 deletion (TAP2-/-) (Courtesy of Peter van Endert, Ph.D., Institut Necker Enfants Malades (INEM), Centre De Medecine Moleculaire, Paris, France).

This mouse monoclonal Anti-TAP2 Antibody, clone 429.4, Cat. No. MABF945 is validated for use in Immunocytochemistry and Western Blotting for the detection of TAP2.
Research Category
Inflammation & Immunology

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TAP2 in 10 µg of Raji cell lysate.

Protein G purified.
Format: Purified
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

429.4, monoclonal

human

immunocytochemistry: suitable
western blot: suitable

IgG1κ

NP_000535

Q03519

ambient

~70 kDa observed. 75.66/72.00 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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