Anti-Puromycin, clone 12D10, Alexa Fluor^® 647 Conjugate Antibody

32160702

NA.41

This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.

Predicted to react with cells from all species when pre-incubated with puromycin.

Evaluated by Immunocytochemistry Analysis untreated and puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected puromycin-incorporated neosynthesized proteins in puromycin treated HeLa cells.

Alexa Fluor^® is a registered trademark of Life Technologies.

Protein G Purified

100

mouse

ALEXA FLUOR^® 647

purified antibody

primary antibodies

12D10, monoclonal

human

0.5 mg/mL

immunocytochemistry: suitable

wet ice

Refer to Cat. No. MABE343 for observed molecular weight information.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2