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Anti-phospho-ULK1/ATG1 (Ser758) Antibody
| 产品编号： | 4112739 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-phospho-ULK1/ATG1 (Ser758) Antibody
Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins. |
| Specificity【特异性】 | This antibody recognizes the ULK1/ATG1 phosphorylated at Ser758. |
| Immunogen【免疫原】 | Epitope: Phosphorylated Ser758 KLH-conjugated linear peptide corresponding to human ULK1/ATG1 phosphorylated at Ser758. |
| Application【应用】 | Research Sub Category Apoptosis - Additional Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot blocked phospho-ULK1/ATG1 (Ser758) in serum-starved A431 cell lysates. Research Category Apoptosis & Cancer Detect phospho-ULK1/ATG1 (Ser758) using this Anti-phospho-ULK1/ATG1 (Ser758) Antibody validated for use in Western Blotting, Peptide Inhibition Assay. |
| Quality【质量】 | Evaluated by Western Blot in A431 and serum starved A431 cell lysates. Western Blot Analysis: A 1:1,000 dilution of this antibody detected ULK1/ATG1 on 10 µg of A431 and serum starved A431 cell lysates.. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control A431 and serum starved A431 cell lysates. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| technique(s) | inhibition assay: suitable (peptide) western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_003556 |
| UniProt accession no.【UniProt登记号】 | O75385 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~120 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
