Anti-phospho Histone H3 (Tyr41) Antibody| ABE566

32160702

NA.41

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle.

Epitope: Near N-terminus
KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3 phosphorylated at Tyr41.

This Anti-phospho Histone H3 (Tyr41) Antibody is validated for use in western blotting, ICC & dot blot for the detection of phospho Histone H3 (Tyr41).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho Histone H3 (Tyr41) in HeLa and NIH/3T3 cells.

DyLight^® is a registered trademark of Thermo Fisher Scientific.

Dot Blot Analysis: 0.5 µg/mL from a representative lot detected phospho Histone H3 (Tyr41) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Evaluated by Western Blotting in colcemid treated HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H3 (Tyr41) in 10 µg of colcemid treated HeLa acid extract.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

DyLight is a registered trademark of Pierce Biotechnology, Inc.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

mouse, human

dot blot: suitable
immunocytochemistry: suitable
western blot: suitable

NP_003520

P68431

wet ice

human ... HIST1H3F(8968)

~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1