Anti-phospho Histone H2B (Tyr37), clone 15 Antibody

32160702

NA.41

Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

KLH-conjugated linear peptide corresponding to human Histone H2B phosphorylated at Tyr37.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho Histone H2B (Tyr37) in mouse MEF cells which were untreated or treated with WEE inhibitor (Mahajan, K., et al. (2012). Nat Struct Mol Biol. 19(9):930-937.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho Histone H2B (Tyr37) in MEF-overexpressing WEE (Mahajan, K., et al. (2012). Nat Struct Mol Biol. 19(9):930-937.).
This Anti-phospho Histone H2B (Tyr37) antibody, clone 15 is validated for use in western blotting & ChIP for the detection of phospho Histone H2B (Tyr37).
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H2B (Tyr37) in 10 µg of HeLa cell lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified antibody

primary antibodies

15, monoclonal

human, mouse

ChIP: suitable
western blot: suitable

IgG1κ

NP_778225

Q8N257

wet ice

~17 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

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12 - Non Combustible Liquids

WGK 1