Anti-phospho Angiomotin Antibody (Ser175)

32160702

NA.41

Angiomotin is a cell surface protein that plays a central role in tight junction maintenance and the uptake of proteins at tight junctions but also appears to regulate endothelial cell response and their migration toward growth factors as well as their response to angiostatin. Thus Angiomotin plays a role in blood vessel formation, cell migration, protein localization, chemotaxis, cellular polarity and many more cell-movement/migration activities. Angiomotin is a component of a multi-protein complex associated at tight junctions that includes ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 proteins. Angiomotin is expressed in endothelial cells and found in placenta, blood vessels and skeletal muscle tissue, as well as at the leading edge of lamellipodia and cellular processes.

This antibody only detects p130 isoform.

Epitope: Phosphorylated Ser175
Linear peptide corresponding to human phospho Angiomotin (Ser175).

Anti-phospho Angiomotin Antibody (Ser175) | ABS1045 is an antibody against phospho Angiomotin Antibody for use in Western Blotting.
Western Blotting Analysis: A representative lot detected phospho Angiomotin (Ser175) in HEK293 transfected with AMOT cell lysate (Dr. Joseph Kissil, Scripps Research Institute, Florida).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

Evaluated by Western Blotting in HEK293 cell lysate transfected with LATS (Angiomotin Kinase).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho Angiomotin (Ser175) in 10 µg of HEK293 transfected with LATS.

T-gel
Format: Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

rabbit

100

purified antibody

primary antibodies

polyclonal

human

mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)

western blot: suitable

NP_001106962

Q4VCS5

wet ice

human ... AMOT(154796)

~150 kDa observed. The calculated molecular weight is 118 kDa, however phospho Angiomotin (Ser175) has been shown as a ~150 kDa band in western blots (Yi, C., et al. (2011). Cancer Cell. 19(4):527-540).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2