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Anti-phospho AMBRA1 Antibody (Ser52)
| 产品编号： | 4112146 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-phospho AMBRA1 Antibody (Ser52)
Activating molecule in BECN1-regulated autophagy protein 1, Ambra, Ambra-1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) interacts with BECN1, likely forming a complex with BECN1 and PIK3C3. AMBRA1 is involved in autophagy by controlling protein turnover during neuronal development, and in regulating differentiation, survival and proliferation of normal cells. |
| Specificity【特异性】 | This antibody recognizes AMBRA1 phosphorylated at Ser52. |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to human AMBRA1 phosphorylated at Ser52. Epitope: Phosphorylated Ser52 |
| Application【应用】 | Western Blotting/Peptide Inhibition Analysis: 2 µg/mL from a representative lot detected phospho AMBRA1 (Ser52) in WB/PIA. Western Blotting Analysis: A representative lot from an independent laboratory detected phospho AMBRA1 (Ser52) in HEK293 cell lysates trated without Torin1 or EBS and demonstrated a loss of signal in HEK293 cell lysates treated with Torin1 or EBS (Nazio, F., et al. (2013). Nat Cell Biol.15(4):406-416.). Research Sub Category Apoptosis - Additional Detect Activating molecule in BECN1-regulated autophagy protein 1 using this rabbit polyclonal antibody, Anti-phospho AMBRA1 Antibody (Ser52) validated for use in western blotting & Peptide Inhibition Assay. Research Category Apoptosis & Cancer |
| Quality【质量】 | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected phospho AMBRA1 (Ser52) in 10 µg of HEK293 cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control HEK293 cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| technique(s) | inhibition assay: suitable (peptide) western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_060219 |
| UniProt accession no.【UniProt登记号】 | Q9C0C7 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... AMBRA1(55626) |
产品说明
| Target description【目标描述】 | ~150 kDa observed. Uniprot describes 6 isoforms from 83 kDa to 143 kDa This protein may be observed at higher molecular weights possibly due to hyperphosphorylation (Nazio, F., et al. (2013). Nat Cell Biol. [Epub ahead of print].). |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
