Anti-Pannexin-1/PANX1 Antibody

32160702

NA.41

Pannexin-1 (UniProt Q96RD7; also known as Innexin) is encoded by the PANX1 (also known as MRS1, PX1) gene (ORF UNQ2529/PRO6028; Gene ID 24145) in human. Pannexin 1 is a high-conductance, voltage-gated channel protein widely expressed in many organs and tissues, including the sensory systems (eye, inner ear, taste buds, and the olfactory epithelium) and both neuronal and non-neuronal cell types. Three pannexin family genes (PANX1-3) have been identified in human and mouse genomes. Pannexin proteins share structural and functional similarities and play important roles in many physiological and pathological processes, such as ATP release, Ca2+ homeostasis, synaptic neurotransmitters release, apoptosis, and immunological response. Pannexin-1 deficiency causes caspase-3 activation and apoptosis induction of hair cells in cochlear spiral limbus (SLM), resulting in hearing loss among Panx1-/- mice. Panx1 is also expressed in the retina, lens and cornea of the eye, where it plays an important role in mediating the crosstalk between immune and neuronal cells. Pannexin-1 channel opening, frequently together with the activation of purinergic receptors by extracellular ATP, is reported to have a great implication in diverse pathological conditions, including ischemia, trauma, HIV infection, inflammation, migraine, and cancer cell growth.

Expected to react with both spliced isoforms reported by UniProt (Q96RD7-1 & Q96RD7-2).

Epitope: C-terminal
GST-tagged recombinant human Pannexin-1/PANX1 C-terminal fragment.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Pannexin-1/PANX1 in human testis and mouse kidney tissue sections.
Research Sub Category
Signaling Neuroscience
Research Category
Neuroscience
Anti-Pannexin-1/PANX1 Antibody is an antibody against Pannexin-1/PANX1 for use in Western Blotting, Immunohistochemistry.

Evaluated by Western Blotting in human cortex tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Pannexin-1/PANX1 in 10 µg of human cortex tissue lysate.

Replaces: AB9886

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Concentration: Please refer to lot specific datasheet.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, mouse, rat

chimpanzee (based on 100% sequence homology), gorilla (based on 100% sequence homology)

immunohistochemistry: suitable
western blot: suitable

NP_056183

Q96RD7

wet ice

human ... PANX1(24145)

~55 kDa observed. 55-58 kDa (membrane glycosylated) and 43 kDa (cytosolic non-glycosylated) expected. 48.1 kDa (isoform 1) & 47.6 kDa (isoform 2) calculated.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1