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Anti-Myelin Basic Protein Antibody, a.a. 82-87
产品别名
Anti-Myelin Basic Protein Antibody, a.a. 82-87
MBP
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | The two major components of CNS myelin are Myelin Basic Protein (MBP) and proteolipid protein (PLP), occuring in roughly equimolar proportions. There are many neurological disorders associated with deficiencies of myelin assembly and structure. For example Multiple Sclerosis is a human disease due to multiple factors but generally characterized by the degradation of the myelin sheath. MBP ususally refers to the 18.5 kDa isoform, which is one of the most abundant proteins of the myelin sheath of adult human and bovine CNS. Genetically, MBP is the product of differential splicing of a single mRNA transcript. In humans there are 4 isoforms, 21.5, 20.2, 18.5, and 17.2 kDa. Thus, MBP are present in multiple isoforms, part of a large family of developmentally expressed, translocatable, and highly post-translationally modifed proteins with multiple binding partners. Structurally, MBP displays a predominately unorganized form with greater than 75% in random coil. Other non-CNS related functions of myelin include insulin induction and glucagon release from pancreatic islets. |
Specificity【特异性】 | Reacts with MBP from all species tested including human, bovine, sheep, rabbit, mouse, rat, guinea pig and chicken. Reacts with MBP from all species tested. It reacts weakly with peptides ending in the phe 91 where the 91-92 phe-phe bond is broken. Synthetic peptide 82-99 reacts very well as does intact MBP. Mapped to the region DENPVV. |
Immunogen【免疫原】 | Epitope: a.a. 82-87 Bovine myelin basic protein |
Application【应用】 | Research Sub Category Neuronal & Glial Markers Neurochemistry & Neurotrophins Immunohistochemistry: A previous lot of this antibody was used on frozen sections. ELISA/RIA: 1:1000 dilution of a previous lot of this antibody was used in ELISA/RIA. Western Blotting: Identifies an 18-20kDa band in westerns. Optimal working dilutions must be determined by end user. Anti-Myelin Basic Protein Antibody, a.a. 82-87 is an antibody against Myelin Basic Protein for use in ELISA, IC, IH, RIA & WB. Research Category Neuroscience |
Quality【质量】 | Evaluated by Western Blot on Mouse brain lysates. Western Blot Analysis: 1:500 dilution of antibody lot detected four isoforms of MBP on 10 µg of Mouse brain lysates. |
Physical form【外形】 | Unpurified Liquid containing 0.1% sodium azide. |
Analysis Note【分析说明】 | Control Brain tissue |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | rat |
antibody form【抗体形式】 | culture supernatant |
antibody product type | primary antibodies |
clone【克隆】 | 12, monoclonal |
species reactivity | sheep, all, mouse, rat, guinea pig, chicken, bovine, rabbit, human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable radioimmunoassay: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2a |
NCBI accession no.【NCBI登记号】 | NM_001025094.1 |
UniProt accession no.【UniProt登记号】 | P02686 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | 18-20 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20ºC from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | nwg |