Anti-Microglia Antibody, clone 25F9

32160702

ANTIGEN DISTRIBUTION:

Isolated cells: Absent on freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 day culture, also positive on some melanoma and carcinoma cell lines. Tissue sections: Kupffer cells, histiocytes (skin), macrophages of the thymus, in the germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma; synovial lining cells, tuberculoid leprosy; no expression in lepramatous leprosy.

Human late stage inflammatory macrophages. The monoclonal is suitable for use on BAL (bronchial lavage fluids) and other lavages. The antigen recognized by MAB1569 is an 86 kDa protein (unreduced conditions), probably a glycoprotein on the cell surface and within the cytoplasm of mature macrophages. It is stable to formaldehyde fixation and paraffin embedding. Enzyme digestion is recommended. The antibody has also been reported to recognize microglia stem cells (X International Congress of Neuropathology, Stockholm, le 11 September,1986.PosterA-701).

Antigen distribution: absent from freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 days in culture, also positive on some melanoma and carcinoma lines. In tissue sections, clone identifies Kupffer cells, histiocytes (skin), macrophages of the thymus, in te germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma;synovial lining cells, tuberculoid leprosy, however no expression in lepramatous leprosy.

Species reactivity is seen in human, rhesus monkey, and pig, other species not tested.

Immunohistochemistry: 1:50-1:200 using human tonsil cryosection. The antibody is reactive on formalin fixed / paraffin sections. Enzyme digestion (proteinase pretreatement) is recommended.

Immunocytochemistry

Optimal working dilutions must be determined by the end user.
Anti-Microglia Antibody, clone 25F9 detects level of Microglia & has been published & validated for use in FC, IC, IH.

Purified immunoglobulin from culture supernatant. Lyophilized from PBS, pH 7.2 containing 5 mg/mL BSA and 0.09% sodium azide. Reconstitute with 500 μL of sterile distilled water.
Format: Purified

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

25F9, monoclonal

human

Chemicon^®

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

IgG1

wet ice

Maintain lyophilized material at 2-8°C for up to 6 months. After reconstitution maintain at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS07

Warning

H312 + H332 - H412

P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

11 - Combustible Solids

WGK 3