Anti-Lysine-specific demethylase 7 Antibody

32160702

NA.41

Lysine-specific demethylase 7, also known as JmjC domain-containing histone demethylation protein 1D, and encoded by the gene JHDM1D/KDM7/KIAA1718, is an important histone demethylase particularly for brain development. JHDM1D specifically demethylates dimethylated ′Lys-9′ and ′Lys-27′ (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 ′Lys-20′ residue (H4K20Me1), thereby playing a central role in histone code. JHDM1D also specifically binds trimethylated ′Lys-4′ of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. JHDM1D also demethylates H3K9me2 in absence of H3K4me3 and JHDM1D has activity toward H4K20Me1 only when the nucleosome is used as a substrate and when not the histone octamer is used as substrate. JHDM1D is located in the nucleus and widely expressed but lowered expression is observed in glial cells and lymphoid cells. JHDM1D demonstrates tumor suppression by regulating angiogenesis factors when overexpression under starvation conditions in certain cancers, thus JHDM1D is being examined as a potential anti-cancer target.

This antibody is expected to cross react with two known isoforms of Lysine-specific demethylase 7 based upon immunogen design.

KLH-conjugated linear peptide corresponding to human Lysine-specific demethylase 7.

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Detect Lysine-specific demethylase 7 using this rabbit polyclonal antibody, Anti-Lysine-specific demethylase 7 Antibody validated for use in western blotting.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Lysine-specific demethylase 7 in 10 µg of HEK293 cell lysate.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

mouse, giant panda, rhesus macaque, canine, marmoset, chimpanzee

western blot: suitable

NP_085150

Q6ZMT4

wet ice

human ... JHDM1D(80853)

~110 kDa observed. Uniprot describes two isoforms at 107 kDa (isoform 1) and 92 kDa (isoform 2).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1